A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method have been developed for the estimation of Trimipramine maleate in various pharmaceutical dosage form like tablet, capsule, gel, etc. The selection of mobile phase as well as stationary phase is finalized after assessing the solubility of Trimipramine maleate in different organic solvent as well on the basis of literature survey. The Trimipramine maleate having anti-depressant activity, it is odourless, white crystalline powder. Also soluble in different organic solvent and sparingly soluble in aquatic buffer.