The study examined the relationship between organizational culture and staff performance in secondary schools in Bukedea District. The study adopted a cross-sectional survey design in which both quantitative and qualitative data were collected. The study objectives were as follows: to determine the relationship between teamwork and staff performance and to determine the relationship between innovation and staff performance in secondary schools in Bukedea District. A sample of 100 employees was selected from the population of 157 respondents. The study's primary informants included deputies, teachers, and head teachers. The sampling methods used included simple random for teachers and census sampling techniques for head teachers and deputies. After data collection, analysis, and presentation, it was established that there is a positive and moderate relationship between teamwork and staff performance, as represented by (r = .530, n = 100, p = 0.000< 0.05). It was noted that staff understand the common goal for their organization, and hence, when an organization sets out the goals, all workers are mandated to abide by them to improve their performance. Similarly, it is concluded that in organizations where staff know each other, every member becomes consistent in their work, and in such a situation, all staff recognize each other for the job well done and staff proactively meet each other’s needs by helping each other. In establishing the relationship between innovation and staff performance, it was found that there is a significant and moderate relationship between innovation and staff performance, represented by (r = .503, n = 100, p = 0.000< 0.05), It was noted that organizations with a strong culture of innovation are different in the marketplace and introduce innovations into products and processes to increase their performance. It is therefore concluded that organizational culture through teamwork and a culture of innovation play a tremendous role in staff performance, and hence secondary schools in Bukedea district need to embrace it for better results. It is therefore recommended that a dynamic and entrepreneurial organizational culture that can help staff stick their necks and take risks needs to be built; teamwork needs to be built and improved; more resources should be allocated to the staff members to make their work easy; and staff members should be encouraged to be innovative. Staff members enjoy working in an environment that encourages creative thinking and personal innovation.