Simple yet powerful tool" perfectly describes hand hygiene that involves washing hands with soap and water or using hand sanitizer. Despite its simplicity, it is incredibly effective in preventing the spread of infections and promoting public health. This action significantly reduces pathogen transmission, making it essential in healthcare settings and daily life. This study evaluates the role of hand hygiene in preventing zoonotic diseases among zoo workers, who are in close contact with animals. The objectives are to assess the importance of hand hygiene in reducing disease transmission, evaluate current practices, and propose recommendations for improvement. Effective hand hygiene requires a systematic approach, including education on its significance, training in correct techniques, ensuring access to soap and sanitizers, promoting awareness through campaigns, and continuously refining strategies. For zoo workers, regular hand washing and using personal protective equipment are vital. This proactive approach can limit the impact of outbreaks, aiding global health security. This study underscores the importance of strict hand hygiene protocols to protect zoo workers, animals, and visitors, ensuring a safer environment for all.