UV-Visible Spectroscopy is the earliest instrumentation techniques for analysis/ evaluation of different types of solvents and substances. In pharmaceutical sector UV-Visible spectroscopy is a fundamental technique for quantifying the concentration of active pharmaceutical ingredients (APIs) in formulations. It comprises the necessary to determine the identity, strength, quality, purity of compounds. The method of analysis is based on measuring the absorption of monochromatic light by colourless compounds is near UV path of spectrum (200-400nm). Radiant energy absorption by materials can be quantitatively described using the general law ‘Beers Law’.The application of Chemometrics in combination with UV spectroscopy for the assay of APIs, Impurities, adulteration issues and degradation products present in pharmaceutical dosage form. (1, 2)