Marx's thought on the division of labor in the era of artificial intelligence requires new interpretation and understanding. The development of artificial intelligence technology has profoundly changed the mode and nature of the division of labor, leading to the disappearance of traditional labor methods and the lowering of occupational barriers, providing a realistic path for the "elimination of division of labor" proposed by Marx. Through the analysis of the concept and historical evolution of the division of labor, drawing on Marx's thought on the division of labor, this article explores in depth the refinement and transformation of the division of labor in the era of artificial intelligence. The article argues that the development of artificial intelligence technology helps to solve the problems brought about by the division of labor in the industrial era, promote the reform of the social division of labor, and promote the free and comprehensive development of human beings.