The leaves of neem (Azadirachta indica) are a rich source of bioactive substances that may have medicinal uses. The purpose of this study was to assess the phytochemical, antibacterial, and antioxidant qualities of the extracted components while also optimizing the extraction conditions for neem leaves. Neem leaf extracts were obtained using a variety of extraction techniques, including solvent extraction, ultrasound-assisted extraction, and microwave-assisted extraction. Chromatographic (HPLC, GC-MS) and spectroscopic (FTIR, NMR) methods were used to characterize the extracts. The DPPH and FRAP assays were used to measure the antioxidant activity, while bacterial and fungus strains were used to test the antimicrobial activity. According to the findings, the greatest quantity of bioactive substances, such as azadirachtin, nimbin, and nimbidin, were extracted using ultrasonic assistance. The extracts' strong antioxidant and antibacterial properties suggested that they might find use in medicine and cosmetics. This research leads to more research and development of neem-based products by offering insightful information about the extraction and assessment of neem leaf bioactive chemicals.