Water, a prime natural resource, a basic human need and is indeed required in all aspects of life and health for producing food, agricultural activity, energy generation and maintenance of environment and a substance of life and development. The physico-chemical characteristics of kalisindh river water. The study conducted in different sites of Jhalawar district, Rajasthan, India. Jhalawar district is located between 23o 45’20” and 24o 52’17” north latitude and 75o 27’35” and 76o 56’48” east longitude. Water body will be selected after the physico-chemical and ecological study of three sites in Jhalawar district of Rajasthan, 1. Teendhaar 2. Munderi 3.Gagron. The collection of water samples during April, 2023 to March, 2024. The water quality parameters namely transparency (12.00-120.00 cm),turbidity (1.1-140TNU),electrical conductivity (100-600µS cm -1), total dissolved solids (235-501 mgl-1), pH (7.80-9.50), dissolved oxygen (5.00-14.00 mgl-1), free carbon dioxide (0-10 mgl-1), total alkalinity (70-301 mgl-1), total hardness (41-150 mgl-1),chloride (15.62-80.94 mgl-1),nitrate (0.008-0.025 mgl-1),nitrite (0.002-0.022 mgl-1),sulphate (3.5-45.00 mgl-1),potassium (1.8-5.4 mgl-1), biochemical oxygen demand (0.50-6.00 mgl-1), chemical oxygen demand (2.50-27.00 mgl-1), ammonia (nil-0.49 mgl-1),sodium (10.81-41.50 mgl-1)and potassium (1.8-5.3 mgl-1) reflects on the pristine nature of the river. On the basis of various parameters studied, kalishindh river in this stretch can be placed under the category of oligosaprobic. The water quality analysis, indicated that the river water pollution free and can serve as a good habitat for many aquatic animals including endangered species.