The study examined microentrepreneurs in Los Baños, Laguna, who engaged with DTI Negosyo Centers in 2024 to assess the following: microentrepreneurs' knowledge, attitudes, and practices regarding the Negosyo Center services, the perceived effectiveness of the services, and the relationship between KAP and service effectiveness. A total of 80 purposely sampled microentrepreneurs across 14 barangays were surveyed using a semi-structured questionnaire. The collected data were analyzed employing descriptive statistics, Pearson correlation, and regression analysis through SPSS. The findings elucidate the relationship between KAP levels and the perceived effectiveness of Negosyo Center services. Findings revealed high awareness of core services such as business registration and advisory support, while familiarity with financial assistance and trade fairs remained moderate. Understanding of services was uneven, with a strong comprehension of registration processes but a limited grasp of financial and networking components. Attitudinally, microentrepreneurs express confidence in service reliability and staff competence, though logistical challenges persist, such as accessibility. Utilization patterns were moderate, with registration services most engaged and networking events the least. Statistical analysis reveals significant correlations between KAP variables and service effectiveness, particularly between engagement and behavioral change. The study proposes targeted, evidence-based strategies to enhance Negosyo Center’s services, which include intensifying microentrepreneurs’ awareness, expanding digital and financial inclusion, customizing capacity-building, and institutionalizing monitoring systems to support resilient, inclusive, and empowered entrepreneurship.