Halophytes, the salt-tolerant plants that thrive in saline habitats, have evolved complex biochemical and physiological mechanisms to withstand high salinity levels that are detrimental to most other plant species. Among these adaptations, enzymatic responses play a crucial role in mitigating salt-induced oxidative stress and maintaining metabolic balance. This study investigates the biochemical adaptations of selected halophyte species, with a specific focus on key enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), which are involved in reactive oxygen species (ROS) detoxification and osmotic regulation. The research was conducted by subjecting halophytes to varying concentrations of sodium chloride (NaCl) under controlled conditions. Enzyme assays were performed to measure activity levels, and the results were analysed to understand the correlation between salinity levels and enzymatic activity. The findings indicate that with increasing salinity, there is a significant upregulation of antioxidant enzymes, suggesting an enhanced defines mechanism against oxidative stress. Moreover, elevated activities of certain metabolic enzymes associated with proline synthesis and ion transport were also observed, highlighting their role in osmotic adjustment and ion homeostasis. This study provides a comprehensive insight into the adaptive enzymatic responses of halophytes, contributing to our understanding of plant resilience under abiotic stress. The outcomes have broader implications for developing salt-tolerant crop varieties through bioengineering and breeding programs. By exploring natural models of salt tolerance, such as halophytes, the research underscores the importance of enzyme-based strategies in improving agricultural sustainability in saline-prone regions.