This study assessed the biochemical, electrolyte, and enzyme parameters in liver and kidney of the New Zealand rabbits following oral exposure. This was done because the field exposure of rabbits and other animals is common occurrence due to the wide spread use of DDVP in pest control in farms. New Zealand rabbits were exposed to varying sublethal concentrations (0.00–0.03 mg/l) of Dichlorvos (DDVP) under controlled laboratory conditions. Electrolyte, metabolites and enzyme activities were measured and the data analyzed for means and standard deviations. Analyses of variance (ANOVA) was applied at the 95% confidence limit to check for variability among treatment groups. Turkey HSD Post HOC test was used to separate means where variability occurred. This was done by the aid of the SPSS® 20.0 statistical tool kit. Result indicate that Liver sodium rose to 88.33 ± 2.02 mg/L, potassium declined to 7.00 ± 0.58 mg/L, calcium from 7.27 ± 0.29 to 5.80 ± 0.17 mg/L. ALT increased to 44.33 ± 6.25 U/L, AST fell to 26.33 ± 1.76 U/L, creatinine dropped to 0.36 ± 0.06 mg/L, magnesium to 0.93 ± 0.03 mg/L, ALP to 103.67 ± 3.84 U/L, T.P to 4.90 ± 0.26 mg/L, ALB to 2.80 ± 0.17 mg/L, and urea to 4.23 ± 0.15 mg/L in comparison with the control. Kidney showed elevated sodium (up to 82.33 ± 3.84 mg/L), reduced potassium (6.33 ± 0.33 mg/L), stable calcium (~7.73 mg/L), increased AST, steady ALT, and reduced magnesium (1.00 ± 0.06 mg/L), ALP (87.33 ± 4.63 U/L), and urea (5.90 ± 0.20 mg/L) compared with the control. These findings suggest DDVP exposure disrupts electrolyte homeostasis and enzyme activities in liver and kidney tissues indicating organ-specific vulnerability to DDVP toxicity compared to the control.