Rasakarpura is a classical Ayurvedic preparation containing mercuric chloride (HgCl₂), traditionally used for its potent therapeutic effects. This study presents a detailed method for the preparation of Rasakarpura following classical Ayurvedic protocols, ensuring safety and quality. The prepared material was then subjected to qualitative and quantitative analyses to confirm its composition and purity. Simple chemical tests and instrumental methods were employed to assess the presence and concentration of mercuric chloride, along with other characteristics important for its efficacy and safety. This work aims to standardize Rasakarpura preparation and provide a reliable basis for its use in clinical practice