Background: Ayurveda explains that the human body is sustained by the tridosha (vāta, pitta, kapha), pañchamahabhūta (five elements), and sapta dhatu (seven tissues). Each dhatu is composed of specific elemental combinations and governed by dosha predominance. Objective: To analyze the correlation between panchamahabhuta and dosha with each dhatu, highlighting their physiological roles and clinical implications. Methods: Classical Ayurvedic texts and modern secondary literature and articles were reviewed to identify the dosha associations of each dhatu with respect to panchamahabhuta Results: Rasa, māṃsa, meda, and majjā are predominantly kapha pradhana with Apa mahabhuta predominance; rakta and śukra reflect pitta influence with Tejobhuta predominance; asthi aligns with vāta with vayu-akasha pradhana. Each dhatu physiological function corresponds to its panchamahabhuta composition and state of dosha Conclusion: Dhatu samya depends on balanced pañchamahabhūta and tridosha. Disturbance in elemental harmony leads to dosha derangement and dhatu duṣṭi. Understanding these correlations enhances clinical reasoning in Ayurveda.