Bartholin’s cyst is a benign fluid-filled swelling caused by obstruction of the Bartholin’s duct. The conventional line of management incision and drainage, marsupialisation, or excision often results in recurrence, postoperative pain, and patient discomfort. In Ayurveda, it can be correlated with Karnini Yonivyapad, a condition characterised by Kapha-Rakta vitiation leading to cystic formations. Jalaukavacharana (leech therapy), one of the Raktamokshana (bloodletting) procedures described by Acharya Sushruta, plays a vital role in managing inflammatory and cystic swellings. This review aims to summarise classical Ayurvedic references and modern scientific studies supporting the therapeutic application of Jalaukavacharana in the treatment of Bartholin’s cyst.