Diabetic nephropathy (DN) is a major complication of diabetes mellitus, characterized by progressive renal damage mediated by inflammatory and fibrotic cytokines such as Transforming Growth Factor Beta-1 (TGF-β1) and Tumor Necrosis Factor Alpha (TNF-α). Current therapeutic strategies are often limited by side effects and incomplete efficacy, highlighting the need for safer, natural alternatives. Tinospora cordifolia (Guduchi), a traditional medicinal plant, possesses potent anti-inflammatory, antioxidant, and antidiabetic properties, making it a promising candidate for targeting molecular pathways involved in Diabetic Nephropathy. The present in silico study aims to evaluate the molecular docking interactions and binding affinity of bioactive compounds from Tinospora cordifolia with key target proteins TGF-β1 and TNF-α, which play a central role in the pathogenesis of diabetic nephropathy.