Background: In Ayurveda, Dosha, Dhatu, and Mala form the foundational elements of the human body, with Pitta Dosha being the primary regulator of thermogenesis, metabolism, and transformation. An imbalance in Pitta leads to various inflammatory and metabolic disorders, emphasizing the need for its accurate functional assessment in clinical practice. Aims and Objectives: To analyse and evaluate the Karma of Pitta Dosha through both subjective and objective parameters, aiming to enhance diagnostic precision and personalized therapeutic interventions. Materials and Methods: Classical Ayurvedic texts, including the Brihattrayi and Laghutrayi, were consulted to understand the Prakrita and Vaikrta functions of Pitta. Relevant contemporary medical literature and physiological markers were reviewed to correlate traditional concepts with quantifiable diagnostic indicators. Discussion and Conclusion: Subjective parameters include clinical history, Prakriti and Vikriti analysis, and sensory observations, while objective parameters involve clinical tests and diagnostic tools assessing sensory, motor, and cognitive functions. Prakrita Karma like Pakti, Ushma, Darshana, Kshut, Thrit, Ruchi, Prabha, Medha, Dhi, Shourya, Tanu Mardava reflect healthy Pitta activity. Conversely, Vaikrta Karma due to Pitta Vṛddhi manifests as Pitavit-mutra-netra-twak, Atikshut, Atitrushna, Daha and Alpanidra while Pitta Kṣhaya manifests as Manda Anala, Sheeta and Prabha-hani. Integrating these traditional and modern approaches allows for a comprehensive understanding of Pitta Dosha Karma, supporting evidence-based diagnostics and standardized Ayurvedic care.