Sambukadi Vati is a traditional Ayurvedic formulation mentioned in Bhaisajya ratnavali undr Grahani roga adhikara indicated in Vataja Grahani. This investigation was undertaken to establish pharmaceutical reproducibility and analytical acceptability of Sambukadi Vati through validation of traditional processing techniques i.e. Shodhana and Marana of Sambuka (apple snail shell) and to establish a Standard Operating Procedure (SOP) for the preparation of Sambukadi Vati using contemporary analytical parameters.