Callus induction and in vitro plantlet regeneration system for safflower (Carthamus tinctorious L) using root, hypocotyls ,first leaf ,cotyledon were optimized by studying the influence on organogenesis of seedling age, media factor, growth regulator and excision orientation. Supplementation medium with auxin and cytokinin ratio >1 enhance growth rate of callus culture growth regulators IAA,NAA,BAP, kinetin in the medium where found effective for callus induction and regeneration in all explants . The BAP-(5mg/l) ,NAA-(3mg/l) (5-7) explants and cotyledonary derived callus more shoots were produced on explants cut from more basal region of cotyledon from 5-7 day old seedling than from older seedlings are more distal cut side MS medium was superior to SH - M and B5. Capitula induction was observed in both callus mediated shoots on cotyledon and shoots on rooting medium with sucrose IAA,NAA and IBA well developed plantlet were transferred to the field.