The rationale of this study was to discover the influence of plyometric training programme on selected physiological variables among inter-collegiate level men volleyball players. To realize this purpose of the study thirty various colleges of virudhunagar district, Tamil Nadu state, India. The subject had past experience of at least three years in volleyball and was randomly selected as subjects. Their age ranged in between 19 and 22 years. The subjects were divided into two groups namely plyometric group and control group. The plyometric group was subjected to plyometric training (for weekly three days monday, wednesday, friday) at evening session for six weeks. Breath holding time, vital capacity and resting pulse rate was selected as dependent variable. After the collection of appropriate data, it was statistically analyzed by using paired‘t’ test. The level of significance was set at 0.05. The result of the present study showed that the plyometric training has significant improvement on breath holding time, vital capacity and resting pulse rate of inter-collegiate level men volleyball players.