Speed, agility, and quickness (S.A.Q.) training has become a popular way to train athletes. For the present study, ninety six players were selected as samples from the qualified teams for the pre-quarter final in the Anna University, Salem in the academic year 2017– 2018. Finally forty football players were randomly selected as subjects for the present study. They were divided into two equal groups. Each group consists of 20 subjects. The age of subjects were ranged from 18-25 years. The researcher had been selected the following variables for the present study: physiological variables namely heart rate, systolic blood pressure and diastolic blood pressure. The data was collected before and after twelve weeks of training. The collected data was analyzed by using t-test and applying Analysis of Covariance (ANCOVA) Technique. The level of significance was fixed at 0.05.The findings of the present study have strongly indicates that 12 weeks of S.A.Q. training have significant effect on selected physiological variables i.e., heart rate, systolic blood pressure and diastolic blood pressure of football players.