This is a cross-sectional descriptive study was carried in Khartoum state, Sudan from 2015-2021 among hairdresser, Field work to evaluate sanitary status of working environment of hairdressing shops was done from September 2019 to January 2020. A sample of 250 dressing shops in 31 administrative units within the seven localities in Khartoum state were enrolled randomly in the study using cluster sample and PPP (proportionate probability procedure) [1, 2]. The data were collected by a standard check list and analyzed by SPSS software version 25. Important health instructions/requirements checked at work place were; posters on HBV, working/dressing coats, Autoclaves and antiseptics for sterilization, good water and soap for cleaning, gloves, first aid kits and cupboard for keeping instruments, locked baskets/containers for other waste and safety measures for flammable/dangerous substances. It was found that hairdressers were not given posters or guidelines on hepatitis B/blood diseases, and there is short in working tools especially gloves, autoclave, instruments/means for treating injuries, cupboards for keeping tools and safety box/special containers for sharps/dangerous substances. Important health instructions/requirements checked were; there were no posters/leaflets on hepatitis B, shorts in working tools such as coats, autoclaves or sterilization devices, gloves, instruments for first aid, cupboards for keeping coats and instruments, safety boxes and locked containers or baskets for keeping sharps and other wastes and safety measures for dangerous/flammable substances. Important conclusions were; there is short in hepatitis B information for hairdressers, short in working tools/instruments and short in cupboards for storing tools, negligence in using personal protective equipment, short in sterilization devices, short in safety measures for first aid and dangerous substances, the strength point was there was rare supervision by health authority to these premises, Important recommendations were; periodic supervision by health authorities to barbers shops and posters on health instructions and safety to be provided to these shops, and expanding studies on blood diseases to the rest of hairdressers not only in Khartoum state, but also in other Sudanese states