This study was designed to investigate the impact of ladder training on selected physical fitness variables among hockey players. Thirty men hockey players were randomly selected from Department of Physical Education, Bharathiar University, Coimbatore. The subject’s age ranged from 21 to 25 years. They were divided into two equal groups. The group 1 is considered as experimental group (ladder training) and group 2 was considered as control group. Pre-test was conducted on Speed, Agility and leg explosive power for both the groups and the reading were carefully recorded in their respective unit as pre-test score. After pre test, experimental group was treated with specific ladder training, for duration of 45 minutes, three days per week for a period of six weeks. The control group was not treated with any special training. After six weeks of training post test was conducted and the reading were carefully recorded in their respective units as post test score. The pre and post test were taken for analysis. The collected data on physical fitness variables due to six weeks ladder training was analysed by dependent ‘t’ test with 0.05 level of confidences. From the results of the study, it was found that there was a significant improvement on physical fitness variables among hockey players.