As we all know pap staining also known as the universal smear is one of the most important procedures in cytology as it is used in gynecologic and non-gynecologic cytology smear. It is one of the most widely used stains in cytology, as it has reduced drastically the diagnosis of cervical cancer by 70% in highly developed countries. Pap smear is very important because it reveals the structure of the cell to be examined. examples include, the cytoplasm, nucleus and all cellular granules.