This article presents new aspects regarding theinfluence of nutrient media on acclimatization of cherry rootstock Krymsk-5 (VSL-2). Experiments are carried out for estimating the effect of nutrient media: MS, mMS DKW, WPM on acclimatization of cherry plantlets. The shoot length and width are measured periodically. The highest mean shoot length of Krymsk-5 in acclimatization were observed in DKW media: 4,55±0,22 cm after 30 days, and followed by118,87±2,56 cm growth after 160days in transferring to the field. The DKW nutrient media is found the most suitable among four nutrient media for acclimatization development of Krymsk 5 explants